FastQCFastQC Report
Mon 27 Feb 2023
SRR3127771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2536648
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276451.0898240512676571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89800.085.2273251
CGTAGGG7000.073.854482
ATAGGGC15850.073.535333
ATAACGG2800.070.560051
TAGAGGG50200.070.497462
GTACGGG6150.070.3064042
TAGGGCA16800.069.095984
TAGACGG3500.068.5440441
AGTAGGG42650.068.21092
GGTAAGG14800.067.381671
AGATAGG14600.067.015941
AGTAAGG14900.066.2980351
CGAAGGG30300.065.9217152
GAGGGAT33850.065.531244
TAAGGGA22150.065.138583
ATAGGGA20500.065.108563
AGAGGGC42100.064.858613
ATAAGGG38250.064.753232
CGAGGGA16700.064.726933
AAGAGGG91350.064.568012
Produced by FastQC (version 0.11.5)