FastQCFastQC Report
Mon 27 Feb 2023
SRR3127770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039103
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357291.1756429446451797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118000.085.4365161
ATAGGGC20950.073.3576353
TAGGGCA19200.072.945184
CGTAGGG8550.071.460492
TAGAGGG56750.069.6496352
AGTAAGG19250.069.6494141
ATAGCGG6250.068.4959261
CGAAGGG29500.068.347722
ATAGGGA30750.068.013713
AGTAGGG52750.067.9815142
ATAAGGG50050.067.141452
TAGGGCG5250.067.140344
ACGGGAT8800.065.690724
GGATAGG22250.065.544321
TAAGGGA27950.065.242643
AAGGGAC31050.065.2374954
GATAGGG50500.064.961012
AGGGATG48700.064.948285
AGAGGGC47950.064.787983
AAGAGGG108600.064.5262762
Produced by FastQC (version 0.11.5)