FastQCFastQC Report
Mon 27 Feb 2023
SRR3127769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369990
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271041.1436335174410017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87200.086.0013051
CGTAGGG6450.073.767482
TAGGGCA16800.069.8221744
ATAGGGC16000.069.780113
AGTAGGG43750.069.452042
TAGGGTA7150.069.180964
GGTAAGG14900.068.6475141
ATAGGGA21700.068.383943
TAAGGGA19850.068.349493
TAGAGGG42650.067.929652
AGTAAGG15650.067.1648251
TAGCGGG12850.066.722392
TAGACGG3550.066.388531
CGAAGGG26000.066.13372
ACGGGTA3000.065.9525154
GTACGGG5750.065.542872
GAATAGG13300.065.210511
GATAGGG37700.065.1028062
ATAAGGG34050.064.748852
CGAGGGA13150.064.483723
Produced by FastQC (version 0.11.5)