FastQCFastQC Report
Mon 27 Feb 2023
SRR3127768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404327
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138610.98702082919434No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG48020.34194315141701326No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG47880.3409462326082173No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC45020.3205806055142428No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT15530.11058677929000867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG3700.086.374622
CGTTTTT46100.086.040071
TAGGGCG2150.078.6886444
ACGGGTA1950.077.1193544
TAGGGTA5100.076.4814154
AGGGAAT19600.075.050185
AGTAAGG8100.074.933971
AGGGATG26450.074.270115
TAGCGGG6600.074.057382
CGAGGGA8550.072.553083
GAATAGG7750.072.246921
AGAGGGC18200.072.041183
GTAGGGT4050.071.942353
TAGGGCA8400.071.6108254
TAAGGGA12950.071.489863
AGGGATC12450.071.343745
AATGCGG1650.071.2902451
AACGGGT3100.071.249893
AGTAGGG21650.070.768282
AGGGCAT13400.070.14375
Produced by FastQC (version 0.11.5)