FastQCFastQC Report
Mon 27 Feb 2023
SRR3127767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676266
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188561.1248811346170597No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG50100.29887857893675585No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC46090.27495636134121915No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG44910.2679169057894153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62600.084.387861
CGTAGGG4100.077.942062
ACGGGTA2550.077.402674
TAGACGG1900.076.7507551
TAGGGCG3300.075.475984
AGTAAGG9050.075.369221
AGGGCAT19050.074.5004355
CGAGGGA13600.073.94723
AGGGATG33850.073.858395
CGGGTAT1850.073.667025
TAAGGGA15950.072.4805153
TAACGGG4550.072.29922
AATGCGG2800.072.241211
AGTAGGG25350.072.113822
ATAGCGG3850.072.088481
TAGGGTA5950.071.873914
CGAAGGG14150.071.4050452
TAGCGGG8100.071.361992
AACGGGT3900.071.0942153
AGGGTAT13850.069.897935
Produced by FastQC (version 0.11.5)