FastQCFastQC Report
Mon 27 Feb 2023
SRR3127766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307067
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131821.0085175434771132No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG42420.3245434243233132No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG41670.31880538641094913No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC37330.2856012736914022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43800.085.537291
CGTAGGG3200.082.4455642
TAGACGG1700.080.391761
TAGGGCG2650.080.001084
AGGGCGT2900.079.404685
CGAGGGA7750.079.026163
AGGGATG26600.077.0287555
ATAGCGG2850.076.0633541
CGAAGGG10050.075.941322
AACGAGG4100.074.712281
TAGGGCA9250.074.360174
GTAGGGT4550.073.515043
GTAGGGC8100.073.284953
AGTAAGG8300.073.244351
CGGGTAT1800.073.102725
AGGGCAT13550.072.832975
AGTAGGG22150.072.3162
ACGGGTA1900.071.907414
GTAAGGG21450.071.820712
TAAGGGA11750.070.968343
Produced by FastQC (version 0.11.5)