FastQCFastQC Report
Mon 27 Feb 2023
SRR3127765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863855
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488961.7073490103374647No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG36880.12877746952970734No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC34260.11962896166181598No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT32410.11316913740395376No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT30060.10496341469802067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149500.088.32241
CGTAGGG9200.077.137282
TAGGGCA21000.076.538864
AGGGATG48750.075.194765
ATAGGGC23350.073.867663
ATAGCGG5100.072.866271
ATAGGGA29550.071.728913
AGTAGGG51400.071.410672
AAGGGAT53850.071.303614
GAATAGG21750.070.7225951
AGGGCAT31250.070.231915
AGAGGGC47050.069.921963
AAGGGTA20350.069.745744
TAGAGGG53500.069.661792
GATAGGG43600.069.4183962
GTAGGGA21600.069.408423
GAGGGAT42400.069.16624
AAGGGCA55450.068.7376254
AGTAAGG19000.068.332161
AAGAGGG107800.068.098492
Produced by FastQC (version 0.11.5)