FastQCFastQC Report
Mon 27 Feb 2023
SRR3127764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469982
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT653701.8838714437135406No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG37120.1069746183121411No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC35090.10112444387319589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT194800.088.6369251
CGTAGGG8550.076.400912
TAGGGCA27700.076.346454
ATAGGGC27700.073.631913
AGGGATG61700.073.0448465
AGGGCAT41500.071.795525
GAATAGG24950.070.693571
AGAGGGC59250.070.592373
ATAGGGA37450.070.52463
TAGAGGG65500.070.169342
AAGGGAT70600.069.627864
AGGGAAT42600.069.3900455
AAGGGCA65450.069.1469044
ACGGGTA3650.068.240014
ATGCGGG15450.068.1348652
TAAGGGA34700.068.123133
AAGAGGG129200.067.655062
AGTAGGG58700.067.570042
ATAGCGG7550.067.2815551
GTAGGGA27600.067.2580263
Produced by FastQC (version 0.11.5)