FastQCFastQC Report
Mon 27 Feb 2023
SRR3127763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665248
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475021.7822731693260814No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG29580.11098404351114793No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT29160.10940820516514786No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC29130.10929564528329072No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT27520.10325493162362377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139900.088.810931
TAGGGCA20350.077.321494
CGTAGGG7100.075.642292
AGGGATG49050.075.402415
AGGGCAT29550.073.315025
AAGGGAT55200.072.884794
TAAGGGA24400.071.6311953
GTAGGGA19600.071.3868943
AGAGGGC45700.070.820453
GAGGGAT40100.070.607084
AGTAGGG46700.070.312742
ATAGGGC22050.070.078283
ATAGGGA25800.069.570223
CGAAGGG27050.068.967752
AAGGGTA20500.068.712454
GAATAGG18900.068.332531
GTAGGGC15600.068.249893
TAGAGGG48600.068.242432
AGAGGGA63750.068.134783
AGGGAAT31600.068.112675
Produced by FastQC (version 0.11.5)