FastQCFastQC Report
Mon 27 Feb 2023
SRR3127762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207667
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118800.9837148816685395No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG56790.47024552297943056No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG49800.4123653291842867No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC48810.40416770517038225No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT20810.17231571285793185No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT17410.1441622566485629No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT15650.12958870284606602No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT14740.12205351309591138No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT13710.11352467195013195No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG13460.11145456487591365No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT13270.10988128349950772No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC12850.10640350361482097No Hit
GGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT12520.10367096227685282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41700.086.2813341
TAACGGG3350.084.169762
ATAGCGG2100.082.8655241
AGGGATG23200.080.012675
TAGGGCG2650.079.799164
TAAGGGA13900.079.1135253
AGGGCTA5650.079.017785
CGAAGGG11600.078.594732
GTAGGGC6300.078.324643
TAGGGCA8700.076.1608054
CGTAGGG2850.075.851232
AAGGGCG5950.075.820384
GAGGGAT21850.075.7047044
AGTAGGG19900.075.5694962
GTAGGGA13250.075.546333
AGGGATT19200.075.14275
GTAAAGG9600.074.95691
TAGGGAT14700.074.804934
AGGGAAT16400.074.7904745
AGTACGG1950.074.768481
Produced by FastQC (version 0.11.5)