FastQCFastQC Report
Mon 27 Feb 2023
SRR3127760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115679
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117701.0549629418497615No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG48650.4360573247322931No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG45690.4095263960332676No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC42430.38030652185798963No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT17640.15810999400365158No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT14940.1339094847173784No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT13770.12342259735999334No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT13140.11777581185986291No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT12810.11481797183598508No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG12360.1107845536216062No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT11880.10648224085960209No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC11710.10495850508972562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT40100.086.132281
CGAAGGG10000.085.712392
AGGGATG19850.081.9166265
TAGGGCA7800.079.698754
TAGCGGG5100.079.414622
AGTAAGG6450.078.8987961
TAAGGGA13950.078.3223953
CGTAGGG2850.077.664982
TAGGGAT13250.077.484144
AAGGGAT26500.076.773284
AGGGATT17450.076.754865
AGTAGGG16150.076.109732
ATAGAGG6850.075.6673361
AGGGAAC12750.075.5614245
TAGAGGG16850.075.463352
TAGGGCG2000.075.3515554
AGGGCAT10100.074.913655
GAGGGAT20000.074.88064
GTAGGGA10950.074.835443
AGGGAAT14400.074.735935
Produced by FastQC (version 0.11.5)