FastQCFastQC Report
Mon 27 Feb 2023
SRR3127758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958450
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201031.0264750185095355No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG37160.1897418877173275No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG36140.1845336873547959No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC31030.15844162475426996No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22760.11621435318746969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67000.084.9586641
AGGGATG35850.076.688665
AATGCGG4300.076.5821
CGTAGGG4250.075.194222
TAGGGCA10450.074.654554
TAAGGGA19700.072.283783
ATAGCGG4450.071.8862841
GAGGGAT27750.071.299024
ATAGGGA23950.070.24933
ATAGGGC14150.069.747293
AAGGGAT41250.069.041974
AGTAGGG32350.068.860242
GAATAGG13600.068.835311
TAGGGAT20700.068.337744
GTAGGGA16750.068.179833
CGAAGGG19600.067.8570252
AGGGAAT23650.067.762245
ACTAGGG9100.067.6541442
GGTAAGG14000.067.540641
AGAGGGA47150.066.881393
Produced by FastQC (version 0.11.5)