FastQCFastQC Report
Mon 27 Feb 2023
SRR3127756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943748
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304641.0348711914199178No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC38690.1314310871718639No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG37890.1287134632448158No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG37790.12837376025393477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95900.085.532351
AGGGATG56650.075.744095
GAGGGAT43400.074.7175144
AGTAAGG21300.072.205571
ATAGCGG5800.072.17131
CGTAGGG7450.071.9149252
TAGGGCA16750.071.265944
ATAGGGC19100.070.864643
AAGGGAT58350.070.232594
ATAGGGA31750.070.014483
AGTAGGG52150.069.8421862
ATAGAGG22150.069.43471
TAGACGG4550.069.257361
AGGGATA38100.069.201545
CGAAGGG30800.069.122272
GAATAGG19200.069.07971
TAGGGTA9750.068.927824
TAGAGGG53550.068.893882
TAAGGGA27000.068.406793
TAGGGAT30450.068.217864
Produced by FastQC (version 0.11.5)