FastQCFastQC Report
Mon 27 Feb 2023
SRR3127755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549255
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407711.1487199426358488No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC40490.11408027881907612No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG38540.10858616808316111No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG36200.10199323520006312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126000.085.238181
AGGGATG70300.074.070035
GAGGGAT55100.073.2655264
TAGGGCA22150.071.7135244
TAGCGGG17100.071.4554442
ATAGGGC26500.071.291583
AGTAGGG62400.069.815732
ATAGGGA38800.069.645683
CGTAGGG10000.069.083682
ATAGCGG6750.068.997011
TAGAGGG64800.068.898012
AGGGAAT48000.068.535395
TAGGGTC8200.068.201074
GAATAGG25100.068.03491
ACGGGAT14700.067.77614
TAGACGG4100.067.6966251
AAGAGGG137150.067.092652
TAAGGGA38550.066.927723
ATAAGGG56050.066.9091342
AGTAAGG25750.066.3175051
Produced by FastQC (version 0.11.5)