FastQCFastQC Report
Mon 27 Feb 2023
SRR3127754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771080
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300591.0847395239401245No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG34210.12345367149270321No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG32890.11869018577594294No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC32370.11681366109964346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93600.085.857021
AGGGATG51100.074.8603065
ATAGGGC16800.073.7552643
GACCGAT2450.072.889618
GAATAGG17200.071.521951
TAGACGG4100.071.274651
GAGGGAT40000.070.670444
TAGAGGG46500.070.619782
ATAGGGA30700.070.593713
TAGGGAT26850.070.538844
AGTAGGG50400.070.016092
AGGGCAT25750.069.533785
AAGGGAT56200.069.077634
AGTAAGG18150.069.076811
TAGGGCA13750.068.871564
AGGGATA34750.068.5648045
TAAGGGA27300.068.513223
ATAAGGG41300.068.4459462
CGTAGGG6800.067.8990552
ATAGAGG17850.067.86131
Produced by FastQC (version 0.11.5)