FastQCFastQC Report
Mon 27 Feb 2023
SRR3127753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782409
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281411.0113897705189998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97750.086.172741
AGGGATG48200.072.837055
TAGGGCA20950.072.4572144
AAGGGAT55300.071.726634
AAGGGCA57600.071.718224
CGAAGGG31100.071.477892
TAGCGGG14400.071.148372
CGTAGGG7550.070.340012
AGGGCAA38350.069.976075
CGGGTAT4050.069.626575
AGTAGGG45100.069.505672
GAGGGAT44750.069.4179154
GGTAAGG17800.069.2264941
AGGGCAT33350.069.052445
GTAGGGT10050.068.273153
AGAGGGC46300.068.210533
ATAGGGA29100.067.506633
TAAGGGC25550.067.3214343
ATAGGGC20150.067.170813
AAGGGCG12950.066.774584
Produced by FastQC (version 0.11.5)