FastQCFastQC Report
Mon 27 Feb 2023
SRR3127752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353450
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365971.0913238605018714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127350.085.368161
CGTAGGG9400.075.495832
TAGGGCA25450.074.0498054
ATAGCGG7000.073.92471
TAGCGGG18050.072.904552
AGGGATG56200.071.665785
AATGCGG7550.071.031811
AGGGCAT40800.070.95585
CGAAGGG32800.070.352812
AAGGGAT67050.069.671474
AAGGGCA67650.069.5398254
ATAGGGC27300.069.031783
AGTAGGG54550.068.751462
AAGGGTA29550.068.546794
AGGGCAA43850.068.163975
AGAGGGC57550.067.943093
TAGAGGG65050.067.624242
AGGGTAT23350.066.620585
AAGAGGG126450.066.231212
ATAAGGG54650.065.959752
Produced by FastQC (version 0.11.5)