FastQCFastQC Report
Mon 27 Feb 2023
SRR3127751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547533
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275871.0828907810026407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95600.084.876841
TAGCGGG12150.076.365782
TAGGGCA20050.076.109614
AGGGATG43800.072.209465
CGTAGGG7200.071.956342
AAGGGCA49850.071.710954
AAGGGAT49150.071.582354
ATAGGGC19000.070.648043
AAGGGTA22500.070.543384
AATGCGG6350.069.753841
AGGGCAT32300.069.547025
TAACGGG8150.069.347782
TAGGGTA8150.069.347784
AGAGGGC45150.069.057233
ATAGCGG5600.068.998521
AGTAGGG43300.068.41822
ATAGGGA30400.068.014243
GGTAAGG17000.067.9095841
AGGGCAA35300.067.89675
TAGAGGG47500.067.722972
Produced by FastQC (version 0.11.5)