FastQCFastQC Report
Mon 27 Feb 2023
SRR3127750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835524
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373241.3162999149363575No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG31830.11225438402214193Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC29410.10371980628624551Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28360.10001678702067061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118100.085.555011
ATAGCGG5150.080.377881
AGGGATG48450.074.398515
AGGGCAT28700.071.722415
AGAGGGC48150.070.5653
GTACGGG6950.070.325232
TAGGGCA19200.070.247044
AAGGGCA47200.069.993984
AGATAGG17700.069.3630751
ATAGGGA27400.068.776673
CGAAGGG28350.068.463592
TAGAGGG53650.068.238642
ATAGAGG22900.067.785931
ATAGGGC19150.067.7310263
AAGAGGG105950.067.6443562
AGTAGGG50050.067.419112
AGGGTAC12150.067.3031855
AGTAAGG17650.067.160961
AAGGGTA21350.066.47474
AAGGGAT50250.066.2131964
Produced by FastQC (version 0.11.5)