FastQCFastQC Report
Mon 27 Feb 2023
SRR3127749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405309
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491371.442952754067252No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36080.10595220580569928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT158000.086.440091
AGGGATG63000.072.436485
AGGGCAT36150.071.3744355
ATAGCGG6750.070.395171
CGTAGGG9600.070.007322
TAGAGGG65100.069.450082
TAGGGCA23950.068.877954
AAGGGAT61000.067.954374
AAGGGCA59950.067.890244
ATAGGGC22850.067.6687853
TAGCGGG16050.066.7634052
TAAGGGA33800.066.7426153
AGTAGGG54450.066.720652
AGAGGGC50900.066.665033
AAGAGGG129900.066.028642
AGGGTAC14650.065.444215
ACGGGTA4100.065.338584
ATAGGGA31500.065.2002943
TAGACGG5200.065.141011
GTAGGGA26400.064.800163
Produced by FastQC (version 0.11.5)