FastQCFastQC Report
Mon 27 Feb 2023
SRR3127748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656868
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367041.3814762344233888No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG27300.1027525642975112Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112150.086.7903441
ATAGCGG4450.075.205381
AGGGATG49900.072.234385
CGTAGGG7500.070.977712
ATAGGGC17950.069.28583
AGGGCAT27000.069.273715
TAGGGCA17650.069.128934
AAGGGCA46250.068.8556754
GTAGGGA19200.068.2100453
AAGGGAT47350.067.7535254
AGATAGG15400.067.642891
AGTAGGG45300.067.491842
AGAGGGC46100.067.342513
AGAGGGT28850.067.275383
AGGGTAC13050.066.9809955
GGATGGC38400.066.820647
AAGAGGG98550.066.779722
GAGGGAT33500.066.655854
TAGAGGG54000.066.388932
TAAGGGA22550.066.224323
Produced by FastQC (version 0.11.5)