FastQCFastQC Report
Mon 27 Feb 2023
SRR3127747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049213
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275590.9038069823262593No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG40330.13226363655146428No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC40170.13173891099113116No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG36740.12049010679149014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86200.084.353421
TAGGGCA19000.073.21324
ATAGCGG5700.072.611981
AGGGCAT32450.071.9780655
ATAGGGC19800.071.204493
GAATAGG18300.068.364551
AGAGGGC49000.068.286573
AAGGGTA23350.068.228234
AAGAGGG112500.067.424322
TAGAGGG54100.067.063412
TAGGGCG6800.067.036944
TAGGGTA10050.066.868464
ATAGGGA26750.066.4078453
AAGGGCA53650.066.2221764
AGGGTAC14050.065.559895
TAGACGG4450.065.5287861
CGAAGGG32500.065.36132
AGTACGG3600.065.323271
AGTAAGG22400.065.089971
AGTAGGG49350.065.0427862
Produced by FastQC (version 0.11.5)