FastQCFastQC Report
Mon 27 Feb 2023
SRR3127746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686389
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368010.9982939944753525No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC41530.11265767123328549No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG39710.10772059052910586No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG39330.10668977148098045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120400.083.830921
TAGGGCA26850.075.0896454
TAGACGG4300.073.3178561
ATAGGGC25150.071.756343
CGTAGGG10900.069.417092
AGGGCAT41200.069.2402345
TAGAGGG70100.069.187672
TAGGGTA12000.068.145164
ATAGGGA32450.067.92433
AAGAGGG133200.067.742942
AGTAGGG63000.066.242922
AAGGGCA71750.065.959084
AGAGGGC58400.065.907993
GTAGGGC20200.065.8418663
TAGCGGG18900.065.646132
AGGGATG49850.065.3333745
ATAAGGG56450.064.771312
AAGGGTA28300.064.5996554
ATAGCGG6850.064.571471
ATAGAGG27150.064.2994841
Produced by FastQC (version 0.11.5)