FastQCFastQC Report
Mon 27 Feb 2023
SRR3127745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849325
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268450.9421529660533634No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG35610.12497696822931746No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC33690.1182385301782001No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG33070.11606257622419344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83650.084.5369341
ATAGCGG4450.075.16751
TAGGGCA20100.074.040624
ATAGGGC20350.072.89963
AGGGCAT31100.071.3242955
TAGGGTA9450.071.266144
CGTAGGG8300.070.3594742
GGTAAGG17000.069.559521
ATAGGGA25100.068.860693
TAGAGGG51800.068.824822
AAGAGGG103700.068.122642
AGAGGGC42400.067.755263
AGGGTAC13450.067.086455
AGTAGGG46250.066.799182
ACGGGTA3950.066.7682344
AAGGGTA21000.066.15794
GAATAGG19600.065.6203161
AGGGATG38550.065.3425755
AAGGGCG13300.065.154614
ATAGAGG20200.065.0705641
Produced by FastQC (version 0.11.5)