FastQCFastQC Report
Mon 27 Feb 2023
SRR3127744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425926
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120620.8459064495633013No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG34300.24054544204958742RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG30530.21410648238407884RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC28130.19727531442725638RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42650.084.823641
CGTAGGG3000.084.605992
TAAGGGA16000.076.674193
ATAGCGG1900.074.2809141
TAGGGCG2650.072.719594
GGTAAGG10550.072.684981
AGGGCAT16150.072.4669955
GAGGGAT24950.072.3392644
AGGGATG27200.072.230535
GAATAGG8500.071.950531
GGGCGAT4850.071.706376
CGAGGGA8500.071.3344653
AAGGGCA26700.071.118654
CGAAGGG11950.070.406662
AGGGATT24450.070.1661155
ACGGGAT6100.070.117264
ATAAGGG21700.069.9634862
AAGGGAT34300.069.7488564
AGGGTAC6850.069.301755
AGGGATC15950.069.250135
Produced by FastQC (version 0.11.5)