FastQCFastQC Report
Mon 27 Feb 2023
SRR3127743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716080
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161450.9408069553866952No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG33200.1934641741643746RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG31900.1858887697543238RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC28040.1633956458906345RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57100.084.264981
CGTAGGG3350.082.76762
AGGGATG32800.077.083535
GACCGAT1000.075.192268
GAATAGG10900.075.0810551
ATAGCGG2450.074.8697051
AGGGCAT20100.073.415325
CGAGGGA11700.073.1035843
GAGGGAT31850.073.038014
CGGGATG7050.072.659195
AGAGGGC26700.072.1648563
CGAAGGG15200.072.038642
TAAGGGA20200.071.888643
GCGGGAT9550.071.846014
AAGGGAT39150.071.062934
TAGAGGG30900.071.0250552
AAGGGCA33050.070.954884
AGGGATT29200.070.653695
ATAAGGG28000.070.32492
AGGGTAC9500.070.24545
Produced by FastQC (version 0.11.5)