FastQCFastQC Report
Mon 27 Feb 2023
SRR3127742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323992
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117010.8837666692850108No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG29370.22182913491924422RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG27690.20914023649689725RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC24550.1854240811122726RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1000.084.829374
CGTTTTT37650.084.012981
CGTAGGG3100.079.052452
TAGGGCG2700.078.545714
TAAGGGA13300.075.8290943
GGGCGAT5000.075.19036
AGGGCAT16100.075.0151755
AGGGATG23150.074.5001145
ACGGGAT6500.073.9538044
CGGGATG5650.073.19415
AAGGGCA25000.072.953254
ATAGGGC9600.071.672963
AGAGGGC22550.071.056873
AGGGCGA6650.070.667585
ATGAGGG21700.070.582552
TAGCGGG5350.070.470912
TAGAGGG21900.070.368352
AGGGCAC12500.070.302935
AAGGGAT31200.070.237984
CGAGGGA8750.070.017893
Produced by FastQC (version 0.11.5)