FastQCFastQC Report
Mon 27 Feb 2023
SRR3127741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044160
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92080.8818571866380631No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG34300.32849371743794054No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG32720.31336193686791297No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC28870.2764901930738584No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12910.12364005516395954No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT12420.11894728777198896No Hit
GGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT10480.10036775973030954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA750.087.728554
CGTTTTT34400.084.4080051
AGGGATG24550.082.317315
AATGCGG2000.080.0029141
CGAGGGA5450.079.335123
TAACGGG2900.077.792592
ACGGGAT4050.077.7488564
AGTACGG1650.077.0081561
ATAGCGG1350.076.691251
AAGGGAT34500.076.285694
CGAAGGG7850.075.438992
GAGGGAT18200.075.402484
GGTAAGG5700.075.131741
AGGGAAT15700.074.8366855
TAAGGGA12400.074.286283
CAGGGAT9500.074.206484
AGGGATC14850.073.423615
AGGGATT16750.073.231835
TAGCGGG5150.073.0092242
GCGGGGT4700.072.9960254
Produced by FastQC (version 0.11.5)