FastQCFastQC Report
Mon 27 Feb 2023
SRR3127740.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127740.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248672
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123430.9884901719586889No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG32500.2602765177724815No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG32190.25779388021834393No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC28660.2295238461341329No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17070.13670523564234643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48200.087.260781
TACGCGG650.086.855341
ACGGGAT5550.081.289994
ACGGGTA900.078.3262944
ATAGCGG1750.077.9630051
AGGGATG31650.077.064165
GGTAAGG6950.076.49311
GGTACGC255.562044E-575.1932457
CGAAGGG12150.075.038522
AATGCGG2800.073.9304351
AAGGGAT38650.073.807084
CGAGGGA7050.073.3267443
AGTAGGG19400.073.158372
AGGGATT22550.072.5256355
TAAGGGA16850.072.515733
AGTACGG1300.072.379451
AGACGGG6650.072.083752
AGGGAAT19250.072.01955
CGTAGGG3550.071.4865342
TAGGGAT15500.070.948464
Produced by FastQC (version 0.11.5)