FastQCFastQC Report
Mon 27 Feb 2023
SRR3127739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971054
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90110.9279607519252278No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG29680.30564726575453066No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG29210.300807164174186No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC26660.27454703857869905No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12850.13233043682431667No Hit
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT11350.11688330412108903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT32950.085.118911
CACGCGG500.084.84711
AGGGATG22200.080.8636865
GCGTAGG1050.080.806761
AATGCGG1850.078.986791
AAGGGAT29300.077.668014
GTACGGG2150.076.699452
GAGGGAT16800.076.562494
TAGACGG1550.076.027871
ACGGGTA506.91216E-1175.3846054
CGAAGGG8650.075.166732
CGGGATA2900.074.5423365
TGTAGGG7400.073.856542
ATAGCGG1150.073.780091
GGTAAGG5950.073.676761
CGTAGGG2250.073.290592
AGGGATT16900.072.020515
GCGGGAT6300.071.794864
AGGGCAT8250.071.772775
AGGGATC12900.071.766155
Produced by FastQC (version 0.11.5)