Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127738.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1460849 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 12693 | 0.8688783029594435 | No Hit |
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG | 3960 | 0.2710752446009136 | TruSeq Adapter, Index 14 (95% over 24bp) |
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG | 3942 | 0.26984308439818216 | TruSeq Adapter, Index 14 (95% over 24bp) |
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC | 3735 | 0.2556732420667708 | Illumina PCR Primer Index 3 (95% over 23bp) |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2619 | 0.17927930949742238 | No Hit |
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT | 1488 | 0.10185857675913117 | Illumina PCR Primer Index 3 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 4425 | 0.0 | 84.60133 | 1 |
CGTAGGG | 465 | 0.0 | 83.88041 | 2 |
AGGGATG | 3580 | 0.0 | 80.33207 | 5 |
ACGGGTA | 195 | 0.0 | 79.5243 | 4 |
TAGGGCA | 965 | 0.0 | 75.965744 | 4 |
TAAGGGA | 1750 | 0.0 | 75.18661 | 3 |
CGAAGGG | 1210 | 0.0 | 74.56779 | 2 |
TAGGGCG | 335 | 0.0 | 74.34497 | 4 |
GAAGGGC | 2095 | 0.0 | 73.79592 | 3 |
GGTAAGG | 920 | 0.0 | 73.101395 | 1 |
GCGGGAT | 695 | 0.0 | 73.022964 | 4 |
AGAGGGC | 2060 | 0.0 | 72.996704 | 3 |
AAGGGAT | 3705 | 0.0 | 72.92898 | 4 |
AAGGGCA | 2880 | 0.0 | 72.1191 | 4 |
GAGGGAT | 2935 | 0.0 | 72.0485 | 4 |
AGGGATT | 2060 | 0.0 | 71.85613 | 5 |
AGGGCAC | 1575 | 0.0 | 71.60629 | 5 |
GGATGGC | 2625 | 0.0 | 71.24826 | 7 |
AGGGCAT | 1825 | 0.0 | 70.80931 | 5 |
GTAGGGC | 775 | 0.0 | 70.33586 | 3 |