FastQCFastQC Report
Mon 27 Feb 2023
SRR3127738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460849
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126930.8688783029594435No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG39600.2710752446009136TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG39420.26984308439818216TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC37350.2556732420667708Illumina PCR Primer Index 3 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26190.17927930949742238No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT14880.10185857675913117Illumina PCR Primer Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44250.084.601331
CGTAGGG4650.083.880412
AGGGATG35800.080.332075
ACGGGTA1950.079.52434
TAGGGCA9650.075.9657444
TAAGGGA17500.075.186613
CGAAGGG12100.074.567792
TAGGGCG3350.074.344974
GAAGGGC20950.073.795923
GGTAAGG9200.073.1013951
GCGGGAT6950.073.0229644
AGAGGGC20600.072.9967043
AAGGGAT37050.072.928984
AAGGGCA28800.072.11914
GAGGGAT29350.072.04854
AGGGATT20600.071.856135
AGGGCAC15750.071.606295
GGATGGC26250.071.248267
AGGGCAT18250.070.809315
GTAGGGC7750.070.335863