FastQCFastQC Report
Mon 27 Feb 2023
SRR3127737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737269
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168100.9676106578773926No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG37850.21787069244889537TruSeq Adapter, Index 14 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG36190.20831546525034408TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC34230.19703338976289797Illumina PCR Primer Index 3 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31440.1809737006761762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5700.088.223072
CGTTTTT60650.085.411731
ATAGCGG3700.076.297541
AGGGATG43700.075.606585
CGAAGGG15850.075.017832
TAGCGGG9550.074.802052
GCGGGAT10400.074.113324
CGAGGGA11500.073.971853
AAGGGAT45800.073.063464
GCGTAGG2900.073.008861
GGATGGC29050.072.8034747
AGGGAAT26650.072.6583945
ACGTAGG3050.072.503511
TAGGGCA12950.072.2219244
AGTAGGG27750.071.808552
GTACGGG4600.071.5176852
TAAGGGA19950.071.381473
TAACGGG5800.071.306312
GTAAGGG28650.071.029142
GAGGGAT35150.070.33094
Produced by FastQC (version 0.11.5)