FastQCFastQC Report
Mon 27 Feb 2023
SRR3127736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347241
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122610.9100821605043197No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG38250.28391356854490024TruSeq Adapter, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG33460.2483594249284278TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC32060.2379678171908367Illumina PCR Primer Index 3 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23160.17190688228757883No Hit
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT14080.10450988353234499Illumina PCR Primer Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG3200.086.8901142
CGTTTTT42950.085.397761
AGGGATG31600.078.521475
ACGGGTA1550.076.011044
AGCGGGA9900.075.688573
CGAAGGG12400.075.630982
GGATGGC24350.075.267467
AAGGGAT36750.074.894
GCGGGAT6900.074.4467544
GAGGGAT26300.074.363654
AGAGGGC19150.074.3201453
CGGGTAT1150.073.555695
GGGCGAT5750.073.555696
AGGGATT20550.073.4065555
AGGGAAT20300.073.384585
AGGGTAT9800.073.368055
AGTACGG2250.073.335711
TACGCGG650.072.529831
AAGGGCA26750.072.23184
TAAGGGA14900.072.1135563