FastQCFastQC Report
Mon 27 Feb 2023
SRR3127735.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127735.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674424
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164720.9837412746114484No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG33240.19851602700391302No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC32070.1915285495191182No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31940.1907521631319188No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18410.10994825683339465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51850.085.753571
ATAGCGG2400.072.537011
GGTAAGG10900.072.087371
AGGGATG23750.071.834335
TAAGGGA15800.071.6884163
AGTAGGG27000.070.32662
AGGGCAT14650.070.257965
TAGGGCA11850.069.802414
AGGGTAC6600.069.786465
TAGGGAC8750.068.750844
ACTAGGG8550.068.714112
AAGGGAC15900.068.575184
TATAGGG13500.068.2376862
AGGGACT20850.068.0753565
ATAGGGC11050.068.052923
TAGGGTA4850.067.831654
ATAGGGA15550.067.7028053
ATTACGG1400.067.215771
GTAGGGA10650.067.0784453
AGAGGGC24550.067.004753
Produced by FastQC (version 0.11.5)