FastQCFastQC Report
Mon 27 Feb 2023
SRR3127734.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127734.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005247
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218511.0896911951495252No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31390.15653931909635072No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31230.15574141240455663No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC28620.14272555949466573No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23200.11569647031014134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66050.083.205061
GGTAAGG11150.073.426711
TAAGGGA20700.072.874013
AGTAGGG35450.069.330352
ATAGGGC15000.069.2371143
AGGGATG29200.069.202785
CGAGGGA11000.068.781423
TAGAGGG39000.068.080352
TAGGGCA14850.068.037754
AGAGGGC31100.067.845953
AGGGCAT20100.067.801595
CGAAGGG19700.067.7472
GAGGGAT27550.067.718434
TATAGGG17350.067.4431462
AAGGGAT35200.066.618654
AAGAGGG69450.066.582612
ATAAGGG30550.066.2985462
ATGAGGG28400.066.188052
AGGGTAT12300.066.096575
TAGGGTA7200.065.9214554
Produced by FastQC (version 0.11.5)