FastQCFastQC Report
Mon 27 Feb 2023
SRR3127733.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127733.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569925
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162511.0351449910027548No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG31090.19803493797474403No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC27980.1782250744462315No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG27770.17688743092822903No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18090.11522843447935412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT52650.083.0644151
GGTAAGG8750.072.7094961
AGGGATG22650.072.618355
CGTAGGG5400.072.416712
TAAGGGA14900.072.094653
AGTAGGG27800.071.688592
TAGGGTA4600.069.64754
GACCGAT1350.069.62148
TAGAGGG30150.068.913732
ATAGGGC11900.068.890093
GTAGGGC9250.068.252343
TATAGGG12800.068.095152
AAGGGTA10950.067.9825444
TGTAGGG12000.067.923382
AGAGGGC23700.067.78923
TAGGGCA11350.067.662224
ATAAGGG23950.067.475032
ATGAGGG25300.067.412822
ATAGGGA16300.067.34773
AGCAGGG29550.067.283632
Produced by FastQC (version 0.11.5)