FastQCFastQC Report
Mon 27 Feb 2023
SRR3127732.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127732.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078492
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175990.8467196409704728No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG36170.17402039555600887No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG31700.15251441910769922No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC28510.13716675358865946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58350.083.6730041
AGTAAGG15000.074.316631
ACGGGTA2150.072.133754
TAGGGTA8450.071.189574
AGTACGG2650.070.997491
TAGGGCG3600.070.494364
CGAAGGG22100.070.178752
GAATAGG14900.069.448891
AAGGGTA13650.069.203254
GGATAGG13900.068.692631
CGTAGGG4800.068.5394742
TAGGGCA13650.068.514664
ATAGGGC13950.068.3920753
AAGGGCA35650.068.02264
TACGGGT1250.067.677843
AAGAGGG74750.067.589812
ATAAGGG31450.066.799132
AGGGATG26650.066.658825
AGTAGGG33250.066.433972
AGGGTAC10050.065.935015
Produced by FastQC (version 0.11.5)