FastQCFastQC Report
Mon 27 Feb 2023
SRR3127731.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127731.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504783
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234720.9370871648362353No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG32640.13031068958867892No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG30140.12032978505523233No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC26340.10515881016439348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76450.084.722541
TAGGGTA10050.074.820994
AGTAAGG18050.073.487661
CGTAGGG6350.073.270822
AGTACGG3150.073.169151
ATAGCGG5600.072.235871
GTACGGG6700.070.846132
TAAGGGA25550.069.345773
TAGGGCA14450.069.2757644
AGTAGGG39900.069.023072
AAGGGCA46700.068.8349154
ATAGGGT13200.068.359183
TATAGGG21850.068.18322
AAGGGCG12100.067.970774
TAGAGGG48000.067.851832
ATAGGGC14700.067.777893
CGAAGGG24600.067.438692
AGGGCAT27050.066.542795
AGAGGGC41450.066.4419863
ATAGAGG18700.066.405621
Produced by FastQC (version 0.11.5)