FastQCFastQC Report
Mon 27 Feb 2023
SRR3127730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933150
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171690.888135943925717No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG31880.1649121899490469No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG28150.14561725680883533No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC26240.13573700954400847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54250.085.31581
AGTACGG3100.074.49931
ATAGCGG3700.073.882921
ATAGGGC12450.073.4239043
AGTAAGG11200.072.802421
CGTAGGG4250.072.065862
AGTAGGG31950.071.380512
TAGGGCA12350.070.9661254
GTACGGG4750.070.431982
TAGGGTA7700.069.762054
AAGGGTA12850.069.304884
TAGACGG2600.068.885531
AAGGGCA33450.068.461324
TAGCGGG9500.068.4479752
TAGAGGG34450.068.252172
AGGGTAA10900.067.258125
CGAAGGG17250.067.197192
ATAGGGA16750.066.952573
ACGGGTA2050.066.657544
TAGTAGG8850.066.570911
Produced by FastQC (version 0.11.5)