FastQCFastQC Report
Mon 27 Feb 2023
SRR3127729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488176
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389501.5654037334979518No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG33090.1329889847020468No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC27810.11176862086926327TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG26570.10678505057520046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112950.086.073581
CGTAGGG7000.077.21492
ATAGGGC18100.076.859393
TAGGGCA16200.072.818644
ATAGGGA23300.072.010553
AGGGCAT25750.070.634625
AGGGATG43400.070.3894355
AAGGGCA44750.069.106154
GAGGGAT31600.068.7129754
AAGGGAT46350.068.444394
TAGAGGG47150.068.182942
ATAGAGG20000.067.964711
TAAGGGA24500.067.716193
AGTAAGG14450.067.70351
ATAAGGG38600.067.5782552
AAGAGGG87700.067.4725952
GAATAGG16600.067.151531
AGAGGGC43400.067.0323943
AATAGGG41750.066.8699952
AGTAGGG40600.066.333042
Produced by FastQC (version 0.11.5)