FastQCFastQC Report
Mon 27 Feb 2023
SRR3127728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969760
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507521.7089596465707666No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG32850.11061499919185389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143850.085.9324951
ATAGGGC20600.079.620893
TAGGGCA21350.077.924544
CGTAGGG9100.074.885112
AGGGCAT33500.074.212955
AGGGATG55700.073.3218465
TAGAGGG60300.072.482722
ATAGGGA29800.070.179883
AAGGGAT55950.070.13834
AGTAGGG46350.069.354592
TAAGGGA29050.069.24153
ATAGAGG26500.069.0471341
AGAGGGC51750.068.8379063
AAGGGTA23100.068.765974
AGTAAGG21000.068.763971
AAGGGCA54950.068.7633744
GAGGGAT40650.068.443154
GGTAAGG17800.068.177591
TAGGGAT25600.067.9251564
GTAGGGC16650.067.743223
Produced by FastQC (version 0.11.5)