FastQCFastQC Report
Mon 27 Feb 2023
SRR3127727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304830
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379451.6463253255120769No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG29030.12595289023485465No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC23790.10321802475670658TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG23550.10217673320808911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110800.087.331091
ATAGGGC17550.075.177393
CGTAGGG6600.074.963812
TAGGGCA15450.073.5012744
GGTAAGG13950.073.317091
AGTAAGG15300.071.468761
AGGGATG41700.070.886015
TAGAGGG43850.070.169752
TAAGGGA24000.069.894843
GTAGGGC13350.069.886013
AAGGGAT43150.069.779264
ATAGGGA23550.069.629723
AGGGCAT22500.069.551685
GAGGGAT31100.067.8772054
AGTAGGG39000.067.659652
ATAGAGG18000.067.5564651
AGGGATC19550.067.546915
TAGCGGG12250.067.3144452
GAATAGG15850.067.204581
AAGGGCA41950.067.170044
Produced by FastQC (version 0.11.5)