FastQCFastQC Report
Mon 27 Feb 2023
SRR3127726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544609
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528162.075603756805073No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT41990.1650155289083706No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT39760.15625190353409896No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC30330.11919316484379329Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT29120.1144380138559598No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG28480.11192289267231233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149000.087.374091
ATAGAGG20000.072.6660161
ATAGGGC17600.071.564983
TAGGGCA16150.071.003324
TAGAGGG49750.069.3396152
AGTAGGG43900.069.051582
ATAGGGA21450.068.141473
AGAGGGC41550.067.8673
GAATAGG17100.067.661541
AGGGCAT26550.066.908325
AGATAGG15600.065.424131
AAGGGCA46500.065.289144
AATAGGG45500.065.177312
ATAAGGG38050.064.96952
AAGGGTA18450.064.953894
AAGAGGG94750.064.879422
GAGGGAT26850.064.411714
AGTAAGG17500.063.427381
TAAGAGG20550.063.39731
TATAGGG22300.063.2258722
Produced by FastQC (version 0.11.5)