FastQCFastQC Report
Mon 27 Feb 2023
SRR3127725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067898
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690572.250954888330707No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT39830.1298283058954372No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT38320.12490636911657428No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32590.10622908584314081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT192100.088.5071261
TAGGGCA22300.072.708564
CGTAGGG9700.071.222452
ATAGGGC23050.070.3427663
ACGGGTA4450.068.6474844
ATAGGGA23550.067.651913
AGGGTAC15150.067.626225
TAGAGGG64150.067.620182
AGTAGGG52750.067.355122
AGAGGGC50550.067.032493
ATAGAGG26250.067.0285341
AGATAGG20050.066.872561
AAGGGCA61300.066.6239854
AAGAGGG116650.066.5574342
AAGGGTA22150.066.4112854
AGGGCAT34700.066.3648155
ATAGCGG8800.066.291291
GAGGGAT36450.066.273044
GAATAGG21750.065.971751
AGGGATG39700.065.701265
Produced by FastQC (version 0.11.5)