FastQCFastQC Report
Mon 27 Feb 2023
SRR3127723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938175
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378441.288010414628128No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121450.087.133231
CGTAGGG9050.075.818952
TAGCGGG15450.071.788862
TAGGGCA21600.071.365154
ATAGGGC23450.071.347813
AGTAGGG54550.071.163742
ATAGCGG7150.071.042221
GTAGGGA26400.070.6740653
GAATAGG20100.070.431871
AGGGCAT33200.069.929815
ATAGGGA31650.069.345243
AAGGGAT59550.069.21244
TAGGGAT26800.068.215164
AGGGATG51300.068.159925
AAGAGGG110000.068.146172
GGTAAGG17200.067.8144151
ATGCGGG15750.067.7358862
AGATAGG18700.066.901961
AGTAAGG21550.066.5658341
AGAGGGC48400.066.514863
Produced by FastQC (version 0.11.5)