FastQCFastQC Report
Mon 27 Feb 2023
SRR3127722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596819
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504601.4029062902525815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT163550.087.6151661
ATAGCGG5950.075.902171
CGTAGGG9200.072.029172
TAGGGAT34550.071.143764
ATAGGGA40700.071.017263
AGTAGGG66050.070.514422
TAGCGGG18050.070.3013462
GTAGGGA34800.069.95743
TAGGGCA26950.069.930844
AGGGCAT41700.069.8767555
ATAGGGC26300.069.693453
AGGGATG64650.069.206285
GAGGGAT54900.068.229024
AAGGGCA70450.068.179384
GGTAAGG21600.068.169561
AAGAGGG134200.067.9051062
AGAGGGC58650.067.873243
CGAAGGG38150.067.7556152
ATGCGGG19300.067.696242
GAATAGG22150.067.53881
Produced by FastQC (version 0.11.5)