FastQCFastQC Report
Mon 27 Feb 2023
SRR3127721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705998
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370031.367443730557081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT120500.086.801621
ATAGGGC19450.073.402633
TAGCGGG13100.071.9361652
TAGGGAT24450.071.4963154
TAGGGCA21000.071.3503954
CGAAGGG26950.070.9832462
GTAGGGA25300.070.956643
AGAGGGC45350.070.235723
GAATAGG19100.070.087821
AAGGGCG13250.070.054974
ATAGGGA30600.069.907383
GGTAAGG17150.069.536691
AGTAGGG52000.069.499332
ATAGCGG4550.069.409831
AATGCGG7200.069.395451
GAGGGAT40750.069.376474
AGGGATG48800.068.9510355
AAGAGGG102750.068.785682
AGGGTAC10950.068.238675
AAGGGAT56100.068.031614
Produced by FastQC (version 0.11.5)