FastQCFastQC Report
Mon 27 Feb 2023
SRR3127720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798257
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285271.0194560399563013No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG43920.15695484724955572No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC40720.1455191571038686No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG40010.14298186335279425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82550.084.672061
ATAGGGC19700.075.630613
AGGGATG50250.075.388235
CGTAGGG9150.074.9983752
TAGGGCA18800.072.999824
ACGGGTA3450.072.202724
GGTAAGG17100.071.793111
AAGGGTA22000.071.568014
TAAGGGA26000.070.320313
GAATAGG18400.070.044031
AGTACGG3850.069.6391
GTACGGG7650.069.428382
AAGGGCG13000.068.692144
CGAAGGG28750.068.33742
ATAGCGG6150.068.069621
GTAGGGC14000.067.815333
AGGGTAC12900.067.7684865
AGTAGGG46700.067.7357942
AGTAAGG21250.067.733091
TAGAGGG54550.066.949352
Produced by FastQC (version 0.11.5)