FastQCFastQC Report
Mon 27 Feb 2023
SRR3127719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337003
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378861.1353301150763127No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC43790.13122553380982876No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG43710.13098579773527325No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG38420.11513324980528936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121950.085.248311
CGTAGGG10650.075.0200652
AGGGATG59450.073.91595
ATAGGGC24750.071.778563
GAATAGG22050.071.254641
CGAAGGG33600.070.217052
TAGGGCA24550.069.491754
TAGACGG4350.069.209541
GAGGGAT47400.068.414614
AGTAAGG23650.068.224171
ATAGCGG7450.068.193471
TAAGGGA31950.068.1064453
TAGAGGG69300.067.88582
ATAGAGG26750.067.8795241
AGTAGGG56150.066.960452
GTACGGG8600.066.671392
AAGGGTA29750.066.5079044
GTAGGGC17250.066.4781343
AAGAGGG119900.066.322262
AGAGGGC51150.065.87973
Produced by FastQC (version 0.11.5)