FastQCFastQC Report
Mon 27 Feb 2023
SRR3127718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562466
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277741.083877795841974No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG37260.14540680734885847No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG35340.13791402500560007No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC34680.13533838107510499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84100.085.927951
ATAGGGC19250.075.630173
AGGGATG48100.075.328025
CGTAGGG9200.072.7223362
GAATAGG16750.071.765161
TAGGGCA17750.070.87294
CGAAGGG26500.070.762722
ACGGGTA3200.070.673824
AGGGCAT27700.070.2373055
ATAGGGA25400.069.560853
GTAGGGA21950.069.332253
TAAGGGA22650.069.269713
AGTAGGG42550.068.985182
GTAGGGC13550.068.152863
AGAGGGC38100.068.138723
ATAGAGG18750.067.629981
ATAGCGG4950.067.6147461
AAGGGAT47000.067.465934
AAGGGTA21350.066.8670354
AGACGGG18050.066.823632
Produced by FastQC (version 0.11.5)